The team was founded in 2011 under the name of Leopard Trek and officially stylized as LEOPARD TREK with Brian Nygaard and Kim Andersen as team managers. The Schleck brothers were under contract with the Danish team Saxo Bank managed by Bjarne Riis through the end of the 2010 season. Several other riders followed the Schleck brothers to the new team, including veterans Jens Voigt, Fabian Cancellara and Stuart O'Grady. Subsequent signings included sprinter Daniele Bennati, Davide Vigano and Joost Posthuma. The team became active at the start of the 2011 cycling season. On 13 December 2010, Jakob Fuglsang revealed that the team would be called Team Leopard, in reference to the management company run by Nygaard. Trek, the bike supplier, confirmed shortly before the team was officially presented that they would be a co-title sponsor, giving the team a full name of "Leopard Trek." Team rider Wouter Weylandt died as a result of a high-speed, downhill crash during the 2011 Giro d'Italia. The remaining riders of Leopard Trek left the competition at the completion of the following day's stage.
2012
For the 2012 season, the team was renamed RadioShack–Nissan–Trek. The reason is that the American ceased racing, and their former sponsors joined the Luxembourg Cycling Project. Johan Bruyneel along with several riders from moved to the new team. The lineup for 2012 was officially confirmed on 5 December 2011. The official UCI name for the team is RadioShack Nissan and it is registered in Luxembourg. While the UCI ProTeam is now named RadioShack–Nissan–Trek, in December 2011 Leopard also launched a UCI Continental Team, consisting mainly of U23 riders, called Leopard-Trek. On 17 July 2012, Fränk Schleck was removed from the 2012 Tour de France by the team during the second rest day after his A-sample returned traces of Xipamide. Team RadioShack–Nissan won the team classification of the Tour de France. Johan Bruyneel stood down as General Manager on 12 October in the aftermath of the publication by the US Anti-Doping Agency of its "reasoned decision" on the Lance Armstrong doping case. On 21 December 2012, Nissan announced that they would cease to sponsor the team, with immediate effect.
2013
During the 2013 Tour de France Team RadioShack-Leopard announced that they would not renew Fränk Schleck's contract, leaving him without a team. It also caused a serious and public rift between his brother Andy Schleck and team management, putting his future with the team into doubt. In September 2013, Chris Horner beat Vincenzo Nibali to win the 2013 Vuelta a España becoming the oldest grand tour winner in history, winning two stages along the way.
2014
On 3 July, the team announced that Samsung would become a new minor sponsor of the team.
2015
On 16 December 2015, the team announced that Italian coffee brand Segafredo had committed to a three-year co-title sponsorship effective 1 January 2016, with the team changing name to Trek–Segafredo.
On 27 June 2017 the UCI announced André Cardoso tested positive for erythropoietin in an out-of-competition control on 18 June and has been provisionally suspended. He had been due to support Alberto Contador in his bid for the 2017 Tour de France, with Haimar Zubeldia taking the empty roster place. In April 2019, Cycling Anti-Doping Foundation confirmed that Jarlinson Pantano had returned an adverse analytical finding for EPO, in a doping test carried out on 26 February. Pantano was immediately suspended by the team.
