With a population of 60,000 Catholics, the diocese makes up about two percent of East Tennessee's total population. The diocese is currently home to 47 parishes and four missions. These parishes are served by 54 diocesan priests, 16 religious priests, 8 extern priests, 24 deacons, 10 brothers, and 33 sisters. Eleven religious institutes are represented in the diocese. Perpetual adoration of the Most Blessed Sacrament is available at many of the parishes and Masses are celebrated in a wide array of languages. This is chosen so that the spiritual needs of all parishioners are met. These languages include English, Spanish, Vietnamese, American Sign Language, and Latin. With the influx of large numbers of Hispanic Catholics looking for work, the diocese faces many new challenges. If the estimates are correct and there are about 50,000 new Hispanic immigrants in the area, their number nearly doubles the area's current official Catholic population. The clergy and laity of the diocese are working to welcome these new neighbors.
While the Catholic Church has been a part of life in East Tennessee for over 166 years, the Diocese of Knoxville was founded in 1988. Before the creation, the area was part of the Diocese of Nashville. Because of his role in the creation of the Diocese, Nashville's Bishop James Daniel Niedergeses, was known as the "Grandfather of the Diocese of Knoxville"
COVID-19 response
On May 6, 2020, Bishop Stika issued a decree for the resumption of public Masses amidst the COVID-19 pandemic, including directives which prohibit the reception of Holy Communion on the tongue.
Sexual abuse cases
On January 1, 2020, the Diocese settled a sex abuse lawsuit involving a man who claimed two priests in the Diocese sexually abused him as a child in the 1990s. Those accused were Monsignor Francis Xavier Mankel and the Diocese of Knoxville's first Bishop Anthony O'Connell; O'Connell previously confessed in 2002 to committing acts of sex abuse before he joined the Diocese of Knoxville as well. The terms of the settlement were not disclosed. Originally filed in the Knox County Circuit Court, both parties involved in the lawsuit agreed to instead settle the lawsuit out of court. Despite denying any wrongdoing concerning the two accused priests, who are both dead, the Diocese agreed to settle due to the financial burden which would have grown by continuing the lawsuit.