RPMI 1640
RPMI 1640, also known as RPMI medium, is a growth medium used in cell culture. RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Memorial Institute, from where it derives its name. A modification of McCoy′s 5A medium, it was originally formulated to support lymphoblastoid cells in suspension cultures, but can also support a wide variety of adherent cells.
It has traditionally been used for growth of human lymphocytes. This medium contains a great deal of phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells, and hybridoma cells.Composition
One liter of RPMI 1640 contains:
- Glucose
- pH indicator
- Salts
- Amino acids
- Vitamins
