Psychophysiological Interaction Psychophysiological interaction is a brain connectivity analysis method for functional brain imaging data , mainly functional magnetic resonance imaging . It estimates context-dependent changes in effective connectivity between brain regions . Thus, PPI analysis identifies brain regions whose activity depends on an interaction between psychological context and physiological state of the seed region.The PPI method was proposed by Friston and colleagues in 1997. The model includes an interaction term between a psychological variable and physiological variable. If the interaction term can explain the brain activation of another brain region after taking into account the main effects of the psychological and physiological variables, then it implies a task-dependent connectivity between the two brain regions. The PPI method is mainly applied to blood-oxygen-level dependent signals measured by functional MRI. The hemodynamic response is slow compared with fast neuronal activities. Therefore, Gitelman and colleagues proposed to first deconvolve the BOLD time series of the seed with hemodynamic response function , so that the resulting "neuronal level" signals could match with the timing of the task design . Before the multiplication , both the psychological and physiological variables should be centered. If an fMRI experiment is designed with multiple conditions, then there will be multiple psychological variables included in the model. In this case, a modeling strategy named generalized PPI was proposed. Traditionally, the PPI analysis was implemented using a seed-based strategy. That is, the PPI term is defined using a pre-defined seed, and a voxel-wise analysis is performed to identify regions in the whole brain that showed task modulated connectivity with the seed region. The PPI method can also be applied to every pair of regions in the brain, so that the whole-brain task modulated connectivity, i.e. task connectome , can be mapped.Implementations Major fMRI data analysis software , including , , and , all have modules for PPI analysis. There is also , which is a MATLAB based toolbox dedicated for PPI analysis.
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