Phosphoglucomutase is an enzyme that transfers a phosphate group on an α-D-glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse direction. More precisely, it facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate.
Biological Function
Role in glycogenolysis
After glycogen phosphorylase catalyzes the phosphorolytic cleavage of a glucosyl residue from the glycogenpolymer, the freed glucose has a phosphate group on its 1-carbon. This glucose 1-phosphate molecule is not itself a useful metabolic intermediate, but phosphoglucomutase catalyzes the conversion of this glucose 1-phosphate to glucose 6-phosphate. Glucose 6-phosphate’s metabolic fate depends on the needs of the cell at the time it is generated. If the cell is low on energy, then glucose 6-phosphate will travel down the glycolytic pathway, eventually yielding two molecules of adenosine triphosphate. If the cell is in need of biosynthetic intermediates, glucose 6-phosphate will enter the pentose phosphate pathway, where it will undergo a series of reactions to yield riboses and/or NADPH, depending on cellular conditions. If glycogenolysis is taking place in the liver, glucose 6-phosphate can be converted to glucose by the enzyme glucose 6-phosphatase; the glucose produced in the liver is then released to the bloodstream for use in other organs. Muscle cells in contrast do not have the enzyme glucose 6-phosphatase, so they cannot share their glycogen stores with the rest of the body.
Role in glycogenesis
Phosphoglucomutase also acts in the oppositefashion when blood glucose levels are high. In this case, phosphoglucomutase catalyzes the conversion of glucose 6-phosphate to glucose 1-phosphate. This glucose-1-phosphate can then react with UTP to yield UDP-glucose in a reaction catalyzed by UDP-glucose-pyrophosphorylase. If activated by insulin, glycogen synthase will proceed to clip the glucose from the UDP-glucose complex onto a glycogen polymer.
Reaction mechanism
Phosphoglucomutase effects a phosphoryl group shift by exchanging a phosphoryl group with the substrate. Isotopic labeling experiments have confirmed that this reaction proceeds through a glucose 1,6-bisphosphateintermediate. The first step in the forward reaction is the transfer of a phosphoryl group from the enzyme to glucose 1-phosphate, forming glucose 1,6-bisphosphate and leaving a dephosphorylated form of the enzyme. The enzyme then undergoes a rapid diffusional reorientation to position the 1-phosphate of the bisphosphate intermediate properly relative to the dephosphorylated enzyme. Substrate-velocity relationships and induced transport tests have revealed that the dephosphorylated enzyme then facilitates the transfer of a phosphoryl group from the glucose-1,6-bisphosphate intermediate to the enzyme, regenerating phosphorylated phosphoglucomutase and yielding glucose 6-phosphate. Later structural studies confirmed that the single site in the enzyme that becomes phosphorylated and dephosphorylated is the oxygen of the active-siteserine residue. A bivalent metal ion, usually magnesium or cadmium, is required for enzymatic activity and has been shown to complex directly with the phosphoryl group esterified to the active-site serine. This formation of a glucose 1,6-bisphosphate intermediate is analogous to the interconversion of 2-phosphoglycerate and 3-phosphoglycerate catalyzed by phosphoglycerate mutase, in which 2,3-bisphosphoglycerate is generated as an intermediate.
Structure
While rabbit muscle phosphoglucomutase has served as the prototype for much of the elucidation of this enzyme's structure, newer bacterium-derived crystal structures exhibit many of the same defining characteristics. Each phosphoglucomutase monomer can be divided into four sequence domains, I-IV, based on the enzyme’s default spatial configuration. Each monomer comprises four distinct α/β structural units, each of which contains one of the four strands in each monomer's β-sheet and is made up only of the residues in a given sequence domain. The burial of the active site in the hydrophobicinterior of the enzyme serves to exclude water from counterproductively hydrolyzing critical phosphoester bonds while still allowing the substrate to access the active site.
Disease relevance
Human muscle contains two phosphoglucomutases with nearly identical catalytic properties, PGM I and PGM II. One or the other of these forms is missing in some humans congenitally. PGM deficiency is an extremely rare condition that does not have a set of well-characterized physiological symptoms. This condition can be detected by an in vitro study of anaerobic glycolysis which reveals a block in the pathway toward lactic acid production after glucose 1-phosphate but before glucose 6-phosphate. PGM1 deficiency is known as CDG syndrome type 1t.
