Mehr-un-nissa Begum was the daughter of Ali Quli Beg, who held the title Sher Afgan Khan. Her mother was Mehr-un-nissa Khanum, daughter of Mirza Ghiyas Beg also known as Itimad-ud-daula. She was also the first cousin of Emperor Shah Jahan's wife Empress Mumtaz Mahal, daughter of Asaf Khan. After Sher Afgan's death in 1607, she and her mother were summoned to Agra by Jahangir to act as ladies-in-waiting to his step-mother, Empress Ruqaiya Sultan Begum, chief wife of the late Emperor Akbar. In 1611, her mother remarried Emperor Jahangir, and became known as Empress Nur Jahan Begum. In 1617, Nur Jahan and her brother Asaf Khan, planned to marry Mihr-un-nissa to Prince Khusrau Mirza, Jahangir's eldest son, and to re-create him the heir apparent, in place of Prince Khurram Mirza. However, Khusrau declined their offer, because he loved his wife, the daughter of Mirza Aziz Koka, who had stuck him through the long years of imprisonment and blindness.
Marriage
When Mihr-un-nissa was about sixteen, Jahangir asked her hand in marriage for his son Shahryar. On 22 December 1620, he sent one lakh rupees in cash and goods as a sachaq to Mirza Ghiyas Beg's quarters, which were accompanied by most of the great Amirs. A large celebration of utmost elaboratness was held at Ghiyas Beg's quarters. Jahangir accompanied by the ladies of the imperial harem also went to his quarters. The marriage took place on 23 April 1621 and was performed at Ghiyas Beg's house. Here again Jahangir accompanied by the ladies of the imperial harem participated in the celebrations. After eight gharis had elapsed of Thursday night, the wedding took place under favorable auspices. Muhammad Sharif Mutamid Khan, who observed the events first hand, believed that Nur Jahan's manoeuvrings were jeopardising the security of the state. On 13 September 1623, Mihr-un-nissa gave birth to the couple's only child, a daughter, named Arzani Begum. Jahangir died on 28 October 1627, and her husband Shahryar ascended the throne at Lahore, as her mother Nur Jahan had desired. Shah Jahan ascended the throne on 19 January 1628, and on 23 January, he ordered the execution of Shahryar, Prince Daniyal Mirza's sons Tahmuras Mirza and Hoshang Mirza, and Prince Khusrau Mirza's sons Dawar Bakhsh Mirza, and Garshasp Mirza.
Last years
Mihr-un-nissa, now a young widow of twenty-two, settled with her mother at Lahore. The two of them lived a simple and austere life. Nur Jahan died in 1645, and was buried beside her husband, Jahangir, in a separate mausoleum, which she had built from the funds she received from Emperor Shah Jahan. After her death, Mihr-un-nissa was also buried beside her mother.