J. Howard Mueller was the son of a Unitarian clergyman and grew up in Illinois. He studied biology at Illinois Wesleyan University with a bachelor's degree in 1912. He was then a chemistry instructor at the University of Louisville for two years before receiving his master's degree in 1914. He became interested in pathology and bacteriology and in 1914 attended a summer course at the Medical Faculty of Columbia University. He stayed there for further studies after receiving a scholarship and received in 1916 his doctorate Ph.D. in pathology. He then became an assistant pathologist at the New York Presbyterian Hospital. In 1917 he was a volunteer at the front in France with a medical unit and was involved in empirical proof of the transmission of trench fever by lice. In 1919 he was discharged as a lieutenant and became an instructor in bacteriology under Hans Zinsser at Columbia University. At Columbia, Mueller did research on the requirements for the growth of cultures of pathogenic bacteria. He wrote: He was the first to succeed in isolating and characterizing methionine, which certain streptococci needed for their growth. In 1923 when Zinnser was appointed as the chair of the department of bacteriology and immunology at Harvard Medical School, Mueller followed him and became an assistant professor there. However, Mueller had to interrupt his work on bacterial metabolism, including work on Rous sarcoma virus with a dispute over William Ewart Gye's theory. In 1930 Mueller began his studies on the nutritional requirements of the diphtheria bacillus. His work with the diphtheria pathogen was also of practical importance for the development of vaccines against diphtheria. In addition to basic research, he always aimed at medical applications to fight infectious agents. In 1944 when Oswald Avery, Colin MacLeod and Maclyn McCarty published their research on bacterial DNA, Mueller understood the meaning and importance of their results and published his viewpoint in 1945 in a review article. Mueller was elected a fellow of the American Academy of Arts and Sciences in 1928 and a member of the National Academy of Sciences in 1945.
Selected publications
pmid=16558960cite journal|doi=10.1084/jem.41.1.159|title=On the Nature of Bacterial Allergies|year=1925|last1=Zinsser|first1=Hans|last2=Mueller|first2=J. Howard|journal=The Journal of Experimental Medicine|volume=41|issue=1|pages=159–177|pmid=19868971|pmc=2130923cite journal|pmc = 533524|year = 1933|last1 = Mueller|first1 = J. H.|last2 = Klise|first2 = K. S.|last3 = Porter|first3 = E. F.|last4 = Graybiel|first4 = A.|title = Studies on Cultural Requirements of Bacteria: III. The Diphtheria Bacillus|journal = Journal of Bacteriology|volume = 25|issue = 5|pages = 509–519|doi = 10.1128/JB.25.5.509-519.1933|pmid = 16559631cite journal|pmid=16560065|year=1937|last1=Mueller|first1=J. H.|title=Nicotinic Acid as a Growth Accessory Substance for the Diphtheria Bacillus|journal=Journal of Bacteriology|volume=34|issue=4|pages=429–41|doi=10.1128/JB.34.4.429-441.1937|pmc=545244cite journal|doi=10.3181/00379727-48-13311|title=A Protein-Free Medium for Primary Isolation of the Gonococcus and Meningococcus|year=1941|last1=Mueller|first1=J. H.|last2=Hinton|first2=J.|journal=Experimental Biology and Medicine|volume=48|pages=330–333cite journal|pmid=16560425|year=1941|last1=Cohen|first1=S.|last2=Snyder|first2=J. C.|last3=Mueller|first3=J. H.|title=Factors Concerned in the Growth of Corynebacterium diphtheriae from Minute Inocula|journal=Journal of Bacteriology|volume=41|issue=5|pages=581–91|doi=10.1128/JB.41.5.581-591.1941|pmc=374722cite journal|author=Mueller, J. H.|title=Nutrition of the single cell: Its application to medical bacteriology|series=Harvey Lecture Series|volume=Volume 39, 1943/44|pages=143–161cite journal|author=Mueller, J. H.|title=The chemistry and metabolism of bacteria|journal=Annual Review of Biochemistry|volume=14|issue=1|year=1945|pages=733-748|url=https://www.annualreviews.org/doi/pdf/10.1146/annurev.bi.14.070145.003505cite journal|pmid=16561199|year=1946|last1=Ley Jr|first1=H. L.|last2=Mueller|first2=J. H.|title=On the Isolation from Agar of an Inhibitor for Neisseria gonorrhoeae|journal=Journal of Bacteriology|volume=52|issue=4|pages=453–60|doi=10.1128/JB.52.4.453-460.1946|pmc=518211cite journal|pmc=440962|year=1950|last1=Aycock|first1=W. L.|last2=Mueller|first2=J. H.|last3=Carroll|first3=F. B.|title=Meningococcus Carrier Rates and Meningitis Incidence|journal=Bacteriological Reviews|volume=14|issue=2|pages=115–160|doi=10.1128/MMBR.14.2.115-160.1950|pmid=15426617cite journal|pmid=13142991|year=1954|author1=Mueller, J. H.|author2=Miller, P. A.|title=Variable factors influencing the production of tetanus toxin|journal=Journal of Bacteriology|volume=67|issue=3|pages=271–7|doi=10.1128/JB.67.3.271-277.1954|pmc=357220