Mattheus was born in Port Elizabeth, where he attended Grey High School. In 2014, he was selected to represent Eastern Province at the Under-18 Academy Week held in Worcester. His best performance of the tournament came in his first match, as he kicked three penalties and five conversions for a personal points haul of 19 points in their 49–31 victory over the Pumas. Later in the same year, Mattheus was also drafted into the squad that competed for their 2014 Under-19 Provincial Championship campaign. He played off the bench in a 17–13 victory over, before starting four of their final five matches of the competition, contributing 18 points with the boot. He joined the Eastern Province Kings academy on a full-time basis for 2015 and started the Under-19 team's opening match of the 2015 Under-19 Provincial Championship season, a 28–15 victory over. However, he lasted just 21 minutes of the match before being suffering a concussion and didn't feature in their next six matches. He made his return as a replacement in their 34–22 victory against and started their 29–10 victory over a week later. He was used as a replacement in their final two matches of the regular season against the s and the s, as Eastern Province finished top of the log, winning eleven of their twelve matches during the season. Mattheus also played off the bench in their 31–15 semi-final victory over Free State, as well in the final, which Eastern Province won by beating the BlueBulls 25–23 in Johannesburg to win the competition for the first time in their history.
Eastern Province Kings
In 2016, Mattheus was included in the squad that played in the 2016 Currie Cup qualification series. He made his first class debut in their 19–14 victory over the on 7 May 2016. It took him just four minutes into his debut to score his first points by kicking his first of four penalties in the match. He also scored a conversion to contribute 14 of his team's 19 points in the win. Big points tallies followed in further games, scoring 15 points with the boot in their 35–all draw against the and 21 points against a through four penalties, two conversions and the first try of his senior career. He finished the competition as the Kings' top scorer with 66 points in his eight appearances.
