MacKay was working for the North West Company sometime before 1791. In 1792 he was transferred to Fort Fork on the request of Alexander Mackenzie. MacKay accompanied Mackenzie on his 1793 overland journey to the Pacific Ocean, the first such journey north of Mexico. From 1793 to 1800 MacKay was probably a clerk in the NWC's Upper English River fur district near Lac La Loche, present-day Saskatchewan. In 1800 he was made a NWC partner and worked in the English River district until 1804. In 1808 MacKay resigned from the NWC and returned to the East to retire wealthy at age 38 in Montreal. This was an indication of the scale of income which partners received in the fur trade.
Pacific Fur Company career
In 1810 Mackay, along with several other retired NWC personnel, such as Donald McKenzie and Duncan McDougall, signed a preliminary agreement with the American businessman John Jacob Astor. Astor intended to establish a new fur trading company to operate in the Columbia River region. MacKay, McKenzie, and McDougall recruited additional workers in Montreal for Astor's company, the Pacific Fur Company. MacKay enlisted a number of people, including Gabriel Franchère, David Stuart, Robert Stuart, and MacKay's own son Thomas, who was 13 at the time. All of these people joined MacKay in his 1811 sea voyage to the mouth of the Columbia River on the Tonquin. During the voyage, enmity developed between MacKay and Jonathan Thorn, captain of the Tonquin. Thorn tried to maroon MacKay and others at the Falkland Islands. Along with Alexander Ross, MacKay was instrumental in founding Fort Astoria in early 1811. MacKay led a trading and exploring party up the Columbia River in May 1811. Then in June 1811 he sailed as supercargo on the Tonquin, and led an expedition that attempted to acquire furs from natives along the coast to the north. In a conflict in 1811 with the indigenous people of Clayoquot Sound, the Tonquin was attacked and blown up. Nearly everyone on board, including Alexander MacKay, was killed. As MacKay's son Thomas had stayed at Fort Astoria, he survived. MacKay's wife Marguerite Waddens MacKay had not journeyed to the west with the expedition. As a widow, she later married John McLoughlin and came to the Columbia River region. McLoughlin cared for Thomas MacKay as his stepson.